Non-alcoholic fatty liver disease (NAFLD) is the most common liver disorder worldwide. The present study aims to investigate the novel efficacy of both insulin and vitamin E against fatty liver induced rats. Rats were divided into three groups: control, fatty liver induced, and fatty liver induced treated group received insulin and vitamin E for ten days. The data revealed a decrease in lipid profile in the treated group in comparison with fatty liver group, also a significant decrease in weight was observed in the treated group compared with the fatty liver group, in addition to a slight improvement in the albumin in the treated group. In conclusion, a combination between both vitamin E and insulin may be useful for ameliorating lipid profile and decreasing the body weight in the fatty liver rats.