The Goal of this study was to identify whether the extract of Liriope platyphylla tuber can induce antiencephalitis. The yield of the hot water extraction was 1.5 times higher than the extract by simple homogenization. When the methanol fractionation after the hot water extract was done, the precipitated pellet in 70% methanol showed the highest antiencephalitis activity. In organic solvent partition after 70% methanol extract, the water phases in hexane, chloroform and ethyl acetate were exhibited higher activities, whereas its activity in butanol partition was divided into water and butanol layers. The eluents from the Sep-Pak R18 cartridge by fraction derived from the butanol and water phases in butanol partition showed the highest activity in the fraction of the hexane: ethyl acetate (5: 5) and ethyl acetate, respectively. It is assumed that the antiencephalitic activity is maintained not only in the hydrophilic materials but also in the hydrophobic ones. Therefore, we suggest that specific metabolites retained in L. platyphylla tuber are a high possibility to be applied as antiencephalitic agents.