Background: Methicillin-resistant Staphylococcus aureus (MRSA) is a prevalent cause of nosocomial and community-acquired infections. Because of this, accurate MRSA detection is essential for both implementing control measures and lowering MRSA endemicity. The current study set out to evaluate the performance of various phenotypic methods with mecA-based PCR for MRSA detection. Methods: This investigation includes a total of 2000 S. aureus isolates. Oxacillin disc diffusion, oxacillin MIC, cefoxitin disc diffusion, and the oxacillin screen agar test were used to assess methicillin resistance, and they were compared to mecA-based PCR. Results: According to Cefoxitin and Oxacillin susceptibility tests, 530 (26.50%) and 302 (30.1%) of the 2000 isolates from our hospital were determined to be MRSA. The Cefoxitin disc diffusion test for MRSA identification has a better correlation with the gold standard PCR approach than any other phenotypic method. Conclusion: Our study demonstrated that the cefoxitin disc diffusion method has a high sensitivity and specificity when compared to other phenotypic methodologies for MRSA detection. As an alternative to PCR for the detection of MRSA, cefoxitin disc diffusion test results for MRSA prevalence are compatible with PCR results for the mecA gene.

Background: Methicillin-resistant Staphylococcus aureus (MRSA) is a prevalent cause of nosocomial and community-acquired infections. Because of this, accurate MRSA detection is essential for both implementing control measures and lowering MRSA endemicity. The current study set out to evaluate the performance of various phenotypic methods with mecA-based PCR for MRSA detection. Methods: This investigation includes a total of 2000 S. aureus isolates. Oxacillin disc diffusion, oxacillin MIC, cefoxitin disc diffusion, and the oxacillin screen agar test were used to assess methicillin resistance, and they were compared to mecA-based PCR. Results: According to Cefoxitin and Oxacillin susceptibility tests, 530 (26.50%) and 302 (30.1%) of the 2000 isolates from our hospital were determined to be MRSA. The Cefoxitin disc diffusion test for MRSA identification has a better correlation with the gold standard PCR approach than any other phenotypic method. Conclusion: Our study demonstrated that the cefoxitin disc diffusion method has a high sensitivity and specificity when compared to other phenotypic methodologies for MRSA detection. As an alternative to PCR for the detection of MRSA, cefoxitin disc diffusion test results for MRSA prevalence are compatible with PCR results for the mecA gene.