All 14 species of the genus Kolopterna Graham known from the Palearctic and Ori­ental regions are revised, and three new species are described: Kolopterna nettae n. sp. from Israel, K. turkmenica n. sp. from Turkmenistan and K. lao n. sp. from Laos. Kolopterna grahami Kostjukov & Khomchenko is synonymized under K. desulcata (Kostjukov). Females are redescribed for Kolopterna nartshukae (Kostjukov) and K. salina Graham, with males being described for the first time for both species. The distribution of Kolopterna aymani Doğanlar, K. kur­djumovi Kostjukov & Yegorenkova, K. nartshukae and K. salina is updated with new records of K. salina from Israel and Jordan, and K. nartshukae from Israel and Cyprus. Two new host records are reported: Baldratia arida Dorchin (Ce­ci­domyiidae) for K. salina and Asphondylia spp. (Cecidomyiidae) for K. nettae n. sp. Molecular identifications for two species K. nettae n. sp. and K. salina Graham are provided. Kolopterna trjapitzini Kostjukov & Kosheleva is ex­clu­ded from Kolopterna, moved to Aprostocetus, as A. mashuk nom. nov. An iden­tification key to females of all known species and to males of part of species of Kolopterna is presented.