Code to replicate the benchmark results in the paper "Image processing tools for petabyte-scale light sheet microscopy data". The code is kept as it is for the benchmarks in a Linux computing cluster. For details, please refer to the readme.txt in the zip file and the paper. It includes a small image (512x1800x500) in Zarr format to generate benchmark data for Tiff and Zarr readers and writers, as well as for deskew/rotation benchmarks. We also included a copy of the PetaKit5D software used for the benchmarks. Please cite our paper (https://doi.org/10.1101/2023.12.31.573734) if you find the software useful for your research: Xiongtao Ruan, Matthew Mueller, Gaoxiang Liu, Frederik Görlitz, Tian-Ming Fu, Daniel E. Milkie, Joshua L. Lillvis, Alexander Kuhn, Chu Yi Aaron Herr, Wilmene Hercule, Marc Nienhaus, Alison N. Killilea, Eric Betzig, Srigokul Upadhyayula. Image processing tools for petabyte-scale light sheet microscopy data. bioRxiv 2023.12.31.573734; doi: https://doi.org/10.1101/2023.12.31.573734