Transdermal drug delivery offers a promising approach to enhance the effectiveness and patient compliance of topical antibiotic therapies. This study focuses on formulating and evaluating Aquasomes as carriers for delivering Mupirocin, a potent antibiotic widely used in dermatology. Aquasomes are nano-sized vesicular structures known for their core-shell composition, designed to facilitate enhanced drug permeation through the skin. The formulation process involved encapsulating Mupirocin within Aquasomes, followed by a comprehensive evaluation of their physicochemical properties, in vitro drug release kinetics, skin permeation capabilities, and stability under varying storage conditions. Various analytical techniques were employed to assess the performance of Aquasomes as a drug delivery system. Results indicated successful formulation of Aquasomes with high entrapment efficiency, ensuring efficient encapsulation of Mupirocin. In vitro studies demonstrated controlled and sustained drug release from Aquasomes, suggesting their potential for prolonged therapeutic effects. Skin permeation studies revealed enhanced drug penetration, underscoring Aquasomes' ability to improve transdermal drug delivery. Stability assessments confirmed that Aquasomes maintained their physical and chemical integrity across different storage conditions.This study provides valuable insights into the application of Aquasomes as carriers for Mupirocin in transdermal drug delivery, potentially enhancing its therapeutic efficacy in treating skin infections and offering a platform for developing user-friendly topical formulations. Further research, including preclinical and clinical trials, is essential to explore the clinical feasibility and safety of Aquasomes in practical dermatological applications. In conclusion, leveraging Aquasomes for transdermal drug delivery represents an innovative strategy to improve therapeutic outcomes and patient adherence in managing skin infections with Mupirocin. Key words: Aquasomes, Mupirocin, Formulation, Evaluation