Background: Smoking-induced hypoxia, inflammation, and oxidative stress result in impairment of hematological parameters. Contradictory evidence is found as far as the effect of smoking on blood cells and indices is concerned. Objectives: The objective of this study is to see the effect of use of tobacco smoking on serum vitamin-B12, S.Iron, S.TIBC and S. Ferritin level and compare it with non-smoker healthy adults. Methodology: Total 300 patients were include in this study, in which patients ranging within age 20-40 years, they were further categorized according to use of tobacco smoking. Group A- This group consist of tobacco smoker patients between ages 20- 40 years. (n=150). Group B-This group consist of tobacco non-smoker patients between ages 20-40 years. (n=150). 10 ml blood was drawn through vein puncture. From all collected blood samples S. iron, S. TIBC, S. ferritine, S. vitamin B12 and Liver function tests were measured. All collected data were analysed statistically to calculate p value to see the difference of significance. Results: The Mean concentration of S. Iron (mg/dL) in smoker group was 162.12 ±48.76 while that of Non-smoker control group 143.09 ±47.86 and the difference among them found to be highly significant. The Mean concentration of S.TIBC (mg/dL) in smoker group was 314.08 ±82.53 while that of Non-smoker control group 230.22 ±79.17 and the difference among them found to be highly significant. The Mean concentration of S. Ferritin (micrograms/lit) in smoker group was 178.38 ±50.02 while that of Non-smoker control group 66.73 ±35.65 and the difference among them found to be highly significant. The Mean concentration of S. Vit. B12 (pg/mL) in smoker group was 288.78 ±108.60 that is low as compared to non-smokers group. Non-smoker control group 459.89 ±165.77 and the difference among them found to be highly significant Conclusion: The results of this study suggest that there were low serum vitamin B12 concentrations in smokers compared with non-smokers, which might contribute to the development of vascular and cardiovascular diseases. It may be concluded that there appears a link between smoking and alterations in hematological parameters.

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