Datasets of enzyme kinetics related to the publication "Deciphering the allosteric regulation of mycobacterial inosine-5′-monophosphate dehydrogenase", published in Nature Communications with DOI: https://doi.org/10.1038/s41467-024-50933-6 Individual files contain raw kinetic reaction data of the mycobacterial IMPDHs (wild type and mutant forms Mycobacterium smegmatis or wild type Mycobacterium tuberculosis) as a function of IMP, NAD+, GTP, ATP, ppGpp and Mg2+ concentration. Individual data sets are presented as time data points of the absorbance at 340 nm in an Excel file with a linked Graphpad graphical link. Detailed experimental conditions are available in the related publication.