Diabetic foot infections typically begin in a wound, most often due to neuropathic ulceration. Diabetic foot infection facing unique challenge of successful treatment because of wide spread of resistant bacteria to many antibiotics. Combination antibiotic therapy could be helpful to combat these resistant bacteria. This basically depends on the microbial etiology and selection of appropriate antibiotics for combination therapy. Using manual E-test method, Minimum inhibitory concentration (MIC) was determined susceptibility to individual antibiotics. Fractional inhibitory concentration index (FICI) was used to determine synergistic effects for combined antibiotics. . Gentamicin susceptibility showed 100%, 20%, 30%, 80% and 70% of Escherichia coli, Klebsiella pneumonia, Proteus spp, Pseudomonas spp, and Staphylococcus aureus, were sensitive, respectively. Ciprofloxacine showed 80%, 30%, 60%, 40% and 90 % resistant to Escherichia coli, Klebsiella pneumonia, Proteus spp, Pseudomonas spp, and Staphylococcus aureus, respectively. Ceftriaxone showed 50%, 40% 60%, 10%, and 70% resistant result against Escherichia coli, Klebsiella pneumonia, Proteus spp, Pseudomonas spp, and Staporhylococcus aureus, respectively. Fractional inhibitory concentration index (FICI) recorded and synergistic effects were determined. Knowledge on the antibiotic synergy pattern of the iso?lates will be helpful in determining the drugs for the empirical treatment of diabetic foot infections.