This repository comprises the intermediate results, data, and the respective script to create it, to be use on the second day of the course The Hitchhiker's Guide to scRNA-seq (08-12/07/2024, iMM, Lisbon, Portugal), focused on integration. File description: data: pbmcref.rds: a Seurat R object of a reference of PBMCs retrieved from the R package SeuratData (v.0.2.2.9001) pbmc3k_panc8.rds: a Seurat R object of two data sets - 3k human PBMCs from 10X Genomics and pancreatic islets from indrop1 - retrieved from SeuratData package (v.0.2.2.9001) jurkat.rds: a Seurat R object comprising three data sets - Jurkat, HEK293T and Jurkat:HEK293T (50:50) - retrieved from 10X genomics and published by Zheng et al., 2017 ifnb.rds: a Seurat R object of two human PBMCs data sets - resting/control and interferon-stimulated - retrieved from the R package SeuratData (v.0.2.2.9001) covid.rds: a Seurat R object of a COVID-19 PBMCs data set from Guo et al., 2020 retrieved from cziscience 01_create_datasets.R: R script used to retrieve and parse all the Seurat R objects mentioned above results.zip: compressed folder with intermediate results used for the hands-on exercises