Introduction: The last few decades have seen the emergence of numerous human and animal infectious diseases worldwide. Among emerging and re-emerging diseases, viral haemorrhagic fevers are a major public health problem. Objective: The aim of this study was to map the distribution of agents carrying Crimean-Congo haemorrhagic fever virus (arbovirus-tica) in the natural region of Middle Guinea. Method: Two types of analysis methods (RT-PCR and ELISA) were used. The prefectures of Mamou, Dalaba and Pita were used as collection areas. Random sampling of different types of animals was used to collect the biomaterial. Results: Out of a total of 789 ticks collected and divided into 229 pools, the genera Amblyomma and Rhipicephalus were encountered. Molecular analysis (RT-PCR) for the detection of virus RNA revealed 4 positive cases (1.8%). Direct enzyme-linked immunosorbent assays (ELISA) for the detection of Ag were positive in only 2 cases (0.8%). Conclusion: We found that the Rhipicephalus and Amblyomma species were the main vectors and reservoirs of the pathogen in Middle Guinea.