SGC Open Notebook Project to Characterize the HMTase NSD3 Exp024 Objective: In a previous experiment (exp023), we show that siRNA-mediated knockdown of NSD3 increases expression of E-cadherin, a marker of epithelial cell identity. To determine which isoform of NSD3 is involved in promoting epithelial to mesenchymal transition, I have designed siRNA that targets either the long or short isoform and again use E-cadherin expression as a marker. Additionally, I have started using A549 lung epithelial cancer cells (https://www.atcc.org/Products/All/CCL-185) as my primary model. This cell line proliferates faster and is more amenable to in vitro experimentation than the H1299 cell line I was using previously.

Funding Acknowledgment: The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.