Aim of Study: To evaluate the Sensitivity, Specificity of Real-time PCR (TrueNAT) assay in case detection of Mycobacterium Tuberculosis in Presumptive Pulmonary Tuberculosis Cases(Culture Positive). Material And Methods: This was a prospective, hospital based cross sectional study carried out in SCB Medical College and Hospital, Cuttack, Odisha for a period of one year, i.e from Sept 2019 –August 2020, on 50 Presumptive Pulmonary tuberculosis patients visiting inpatient and out-patient department of Pulmonary Medicine. Statical analysis of the data was conducted with Statistical Package for the Social Sciences (SPSS) VERSION 20.0. Results: Considering culture as the gold standard test the results of TrueNAT (RT PCR ) was compared for Pulmonary tuberculosis specimens in present study. Performance of Real-time PCR (TrueNAT ) assessed against Culture in this study, Sensitivity was 92.11%, Specificity was 91.67%. Conclusion: Our study concludes that Real Time PCR (TrueNAT) has shown good results and has high sensitivity and specificity for detection of Mycobacterium tuberculosis in Presumptive Pulmonary tuberculosis patients.

Aim of Study: To evaluate the Sensitivity, Specificity of Real-time PCR (TrueNAT) assay in case detection of Mycobacterium Tuberculosis in Presumptive Pulmonary Tuberculosis Cases(Culture Positive). Material And Methods: This was a prospective, hospital based cross sectional study carried out in SCB Medical College and Hospital, Cuttack, Odisha for a period of one year, i.e from Sept 2019 –August 2020, on 50 Presumptive Pulmonary tuberculosis patients visiting inpatient and out-patient department of Pulmonary Medicine. Statical analysis of the data was conducted with Statistical Package for the Social Sciences (SPSS) VERSION 20.0. Results: Considering culture as the gold standard test the results of TrueNAT (RT PCR ) was compared for Pulmonary tuberculosis specimens in present study. Performance of Real-time PCR (TrueNAT ) assessed against Culture in this study, Sensitivity was 92.11%, Specificity was 91.67%. Conclusion: Our study concludes that Real Time PCR (TrueNAT) has shown good results and has high sensitivity and specificity for detection of Mycobacterium tuberculosis in Presumptive Pulmonary tuberculosis patients.