The present investigation deals with the anti-mycobacterial prospective value of Andrographolide against Mycobacterium canetti, commonly regarded as smooth variant of the tuberculosis. The bioactive compound andrographolide was extracted from the leaves of Andrographis paniculata. Employing different techniques viz. Zone of Inhibition, Disc Diffusion and Turbidometric Method, the MIC of andrographolide varied between 100 and 11.11μg/ml against the test organism (Garg & Shrivastava, 2013). The result suggested andrographolide compound to be a potent antibiotic against M. canetti, as good as any standard drug like isoniazid and fluoroquniolones.