Design of microfluidic device (Kolnik et al.). 33 cuboid culture chambers are connected to a main perfusion channel that runs between ports 1 and 2. A separate air channel between ports 3 and 4 allows the application of a temporary vacuum at the PDMS interface to draw fluid from the main perfusion channel into the culture chambers. Ports 57 comprise the DAW dynamic stimulation generator. e chambers. Upon the application of a vacuum in the air channel at time 0, fluid containing cells is rapidly drawn into the culture chambers and fills the traps within 2 min, at which point the vacuum is turned off.