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ZENODO
Software . 2018
License: https://opensource.org/licenses/MIT
Data sources: Datacite
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ZENODO
Software . 2018
License: http://opensource.org/licenses/MIT
Data sources: ZENODO
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ZENODO
Software . 2018
License: https://opensource.org/licenses/MIT
Data sources: Datacite
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Macro for quantification of GUV diameter, membrane fluorescence intensity, and FRET

integration_instructionsResearch softwarekeyboard_double_arrow_right Software 21 Jun 2018Publisher:ZenodoFunded by:NIH | Core A: Laser Fluorescenc..., NIH | Structural Dynamics of th...
Authors: Witkowska, Agata;

doi: 10.5281/zenodo.1249321 , 10.5281/zenodo.1249320

Macro for quantification of GUV diameter, membrane fluorescence intensity, and FRET

Abstract

Macro for quantification of GUV diameter, membrane fluorescence intensity, and FRET v1.0.0 https://doi.org/10.5281/zenodo.1249321 based on GUV membrane linearization macro, https://doi.org/10.5281/zenodo.376618 This package contains: FRET_analysis_macro.ijm This macro was developed by Agata Witkowska for the study "A convenient protocol for generating giant unilamellar vesicles containing SNARE proteins using electroformation", Witkowska, Jablonski, and Jahn, 2018, Scientific Reports https://doi.org/10.1038/s41598-018-27456-4 This Fiji macro can be used for automatic calculation of FRET donor signal recovery upon acceptor photobleaching in lipid mixing experiments with GUVs (as in Witkowska & Jahn, 2017, doi:10.1016/j.bpj.2017.03.010; and Witkowska, Jablonski, and Jahn, 2018, doi:10.1038/s41598-018-27456-4) in multiple microscopy image stacks (1 GUV on image present). It saves list with membrane fluorescence intensity over time for each image and a Log file with % donor change after photobleaching, file name, and GUV diameter.

Related Organizations
Keywords

Fiji, macro, GUV, fluorescence microscopy, ImageJ

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selected citations
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This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
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