Ozone (O3) is a stratospheric layer that plays important role in providing support to humans for their survival. It is an essential factor for many global, biological and environmental phenomena. There are many situations where human activities have significant effects on the environment. Ozone layer damage is one of them. The objective of this paper is to review the origin, causes, mechanisms and bio effects of ozone layer depletion as well as the protective measures of this vanishing layer. The chlorofluorocarbon and the halons are potent ozone depletors. One of the main reasons for the widespread concern about depletion of the ozone layer is the anticipated increase in the amounts of ultraviolet radiation received at the surface of the earth and the effect of this on human health and on the environment. The prospects of ozone recovery remain uncertain. However, the future behaviour of ozone will also be affected by the changing atmospheric abundances of methane, nitrous oxide, water vapour, sulphate aerosol, and changing climate. The data regarding effect of ozone depletion on human was reviewed and compiled as a review paper from various published articles of international reputed journals, annual/environmental reports of recognized organization and e-books. This study clearly pointed out towards the alarming rate of how the ozone layer was being depleted and given some needful solution. The current situation of ozone depiction demands urgent remedial measures to protect lives on this earth.