Abstract Myocardial infarction (MI), also referred to as a heart attack, is a condition that affects the blood vessels that supply the heart muscle, namely, the coronary arteries. The cardiac troponins have emerged as the preferred cardiac indicators for individuals with ACS, outperforming CK-MB and myoglobin in terms of clinical significance. The aim of this study was to assess the ratio of Creatine Phosphokinase MB and Troponin level and its ratio in patients with MI among Acute Coronary Syndrome patients. Methods. The study was carried out using a descriptive research design and a quantitative research technique. A total of 60 patients with acute coronary syndrome were the subjects of the data collection in the month of May 2023. The results showed that the ratio between levels of CPK-MB/Troponin was significantly elevated when compared with MI patients (15.53+4.79). The ratio of level of creatine phosphokinase-MB and troponin was found to be statistically significant among ACS patients.