Introduction: the aim of this in vitro study was to evaluate push-out bond strength and interfacial adaptation to dentine wall of EndoSequence BC (BC) and MTA Fillapex sealers compared to AH Plus sealer (AH+). Methods: sixty-six human extracted single rooted teeth with single root canal were selected and the crowns were removed at the level of CEJ to standardized the roots length (16 mm). Samples were instrumented using ProTaper universal NiTi rotary system up to size F5. After irrigation and smear layer removal samples were randomly divided according to tested sealer used into three groups of 22 sample each, and then obturated using lateral compaction technique and stored for 1 week before testing. Each group was subdivided according to test into two subgroups of 11 sample each, push-out test or interfacial adaptation. Data were statistically analyzed by one-way ANOVA and Tukay?s post-hoc test (P≤0.05). Results: AH Plus has a statistically significant higher bond strength value than each of EndoSequence BC group and MTA Fillapex group. Regarding interfacial adaptation there was no statistically significant difference between the three sealers. Conclusion: EndoSequence BC bioceramic sealer, MTA Fillapex MTA based sealer showed their inferiority regarding push-out bond strength when compared to AH Plus resin based sealer. All of the three sealers showed different grades of gap width along the whole length of root canal wall.