Objectives: To measure the frequency of UTI causing organisms and to measure the frequency of UTI causing organisms among male and female patients. Materials and methods: It was a cross sectional study conducted at tertiary care hospitals of the Lahore. Duration of study was six months (August 2017 to Jan 2018). There were 300 patients included in this study. Patients coming at Urology department with urinary tract infections problems were included in the study through purposive sampling. Patients urine for culture was sent for examination. Data was collected through structured questionnaire. Results: All the 300 patients with UTI with positive urine for culture were included in this study. There were 192 (64%) female patients with positive urine for culture and 108 (36%) were male patients having positive urine for culture. Patients included in the study were from 21 to 70 years of age group. Majority of the cases included in the study with positive urine for culture were from 21 to 35 years of age group. Microorganisms identified from urine culture were Klebsiella pneumonea, Escherichia Coli, Staphylococcus sp., Proteus sp., Candida and Pseudomonas. Conclusion: Urinary tract infections are more prevalent among women as compared to men and E. coli is the more prevalent microorganism both among males and females. Keywords: Urinary tract infections, isolated microorganisms, Patients.