Background and objectives: Vitamin D involved in various conditions including signaling pathways that affects the numerous cellular processes. However, according to the recent observations, low level of serum vitamin D is associated with tumorogenesis. Materials and Methods: The study includes 426 individuals of solid malignancies along with age and sex matched healthy controls (n= 426). The serum vitamin D level was estimated by electrochemiluminescence method and the data analyzed statistically. Results: The mean of serum vitamin D in solid malignancies and control groups were 15.53ng/mL and 18.15ng/mL respectively. Vitamin D deficiency was found in the individuals of 380 cases of solid tumors (89.2%) and 355 of the control group (83.3%) and representing the risk association towards the disease (OR=1.65). The Sub group analysis also revealed that low vitamin D levels, i.e., less than 10 ng/mL was observed in all other categories of solid malignancies like breast, lung, gynecological, prostate, gastrointestinal, head & neck, sarcomas (bone & soft tissue) and various other solid malignancies. Conclusion: The serum vitamin D deficiency is significantly associated with all the categories of solid tumors in comparison to control and maintaining the adequate levels may reduce the risk and their associated overall outcomes.