Asphalt roadway is a complex structure usually consists of surface, the base and subbase courses on a subgrade. A complete bond between layers will made the pavement work as a single composite structure. A slippage is the one most common distress due to poor bonding among pavement layers which decrease the life of overlay pavement structure. A tackcoats was a use of an asphalt emulsion between a current bituminous surface and a newly constructed bituminous overlay and usage to raise the bond between bituminous layers (two layers). This study is a try to estimate bond strength at the interface among pavement layers by performing laboratory tests. FDOT test method use to evaluate the bond between bituminous layers, to carry these objective cylinder specimens with 101.6 mm diameter need set using normal Marshall Process firstly for the fundamental layer, follow in application of tackcoat and lastly overlay with the upper layer in the similar mould in a fitting manner. After the samples tested it is detected interface bond strength depend on tack coat type and rate.