The present study described the effect of waste water used for irrigation purpose on the anatomical characters of plants. This waste water comprises of municipal, industrial and dairy waste containing organic matter, Ca++, Mg++, Nitrogen, Phosphorus and Potassium. Anatomy of four wild plants i.e. Trianthema portulacastrum L., Amaranthes viridis L., Portulaca oleracea L. and Persicaria barbata L. was studied. It was found that Trianthema portulacastrum L. and Portulaca oleracea L. were directly affected and the size of vascular tissues and cortex reduced many times as compared to control, while on the other hand Amaranthes viridis L. and Persicaria berbata L. showed high growth than control.