Introduction & Objectives:The canine population is remarkably abundant in Malagasy communities, with the majority of dogs roamingfreely without any restriction. Given the country’s limited investment in the health sector, this study aims toinvestigate dogs as indicators of potential zoonotic pathogen circulation, contributing to the enhancement ofearly warning systems. Rift Valley fever virus (RVFV) and West Nile virus (WNV) were considered as first models.Methodology:Blood samples were collected from dogs in the Ifanadiana and Fianarantsoa I districts and analysed at the VirologyUnit of the Institut Pasteur de Madagascar. ID Screen WNV ELISA Competition Multi-species kit was used for thedetection of antibodies directed against Flaviviruses and ID Screen RVFV ELISA Competition Multi-species kit forthe detection of antibodies directed against RVFV. A first identification of factors influencing dogs’ exposure tothese pathogens was then carried out using logistic regression.Results:These screenings revealed a flavivirus seroprevalence of 56.55%, significantly higher in rural areas (410/577)than in urban areas (56/247): p = 2.2e-16. RVFV seroprevalence was 3.39%, notably higher in rural areas (27/578)than in urban ones (1/247): p = 0.001. Dogs’ exposure to these pathogens increased with age, suggesting anendemic transmission.Further serological analysis based on Luminex technology will enable to distinguish results specific to WNV fromother flaviviruses. Furthermore, calculating force of infection and comparing the results with human serologicaldata in the same study area will enable to assess dogs’ utility as sentinel animals for monitoring these zoonoticpathogens.