Development of ferret immune repertoire reference resources and single-cell-based high- throughput profiling assays
Development of ferret immune repertoire reference resources and single-cell-based high- throughput profiling assays
This UDEL iso-seq data are included for supporting evidence but were not used to identify transcripts (i.e. VDJ sequences) due to low sequencing quality. UDEL_8-5-20_primer_IG.fasta contains ferret splenocyte sequencing (three individual ferrets) and UDEL_6-12-20_IG.fasta Ferret lymph node sequencing (8 -12 samples). All ferrets were female and 4-6 months in age.
We performed long read transcriptome sequencing of ferret splenocyte and lymph node samples full-length, non-chimeric circular consensus sequencing (CCS) reads to obtain over 120,000 high-quality immunoglobin (Ig) and T cell receptor (TCR) transcripts.
Iso-Seq, Immune Repertoire, Circular Consensus Sequence, Pacific Biosciences
Iso-Seq, Immune Repertoire, Circular Consensus Sequence, Pacific Biosciences
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