This study aimed to evaluate and compare the mineral acquisition and color regain potency of synthetic nano hydroxyapatite, nano fluoroappatite, natural nano hydroxyapatite and human enamel nano powder on enamel white spot lesions. Materials and Methods: 24 human premolars were used. Ca and P mineral content by EDAX, CIE L* a* b* and ∆E* color parameters by spectrophotometer were measured at three levels of the study. At each level, ESEM evaluated the surface morphology of the samples. Results: Ca wt % revealed a significant loss after demineralization for CG and significant increase for RG1 and RG3 following the remineralization. P wt % showed a significant increase in RG2. Color measurement showed significant degrees of color change ∆E, after demineralization, as well as L* a* and b* values that showed some degree of color recovery after remineralization. ESEM showed recovery of enamel surface among the remineralized groups. Conclusions: 1-Quantitative element analysis by EDAX is accurate, simplified technique to assess changes in mineral content. 2-All the nano-sized apatite remineralizing materials showed the capability to remineralize the artificial white spot lesions. 3-The remineralizing efficacy of all the nano-sized apatite materials was the same. 4-Color retrieval was efficient to some extent among materials.