Background: Group B-streptococcus is a kind of bacteria that can be present in the vagina of pregnant ladies with a liability to be inhaled by the neonate though its passage in the birth canal during labour with a possibility of neonatal mortality and morbidity ranges from simple RDS* to death, screening all pregnant women for the presence of GBS* help to improve neonatal outcome. Objective: The study was planned to show the effect of group of B-streptococcus on neonatal outcome in normal vaginal delivery. Materials and methods: This is a prospective study counted on retrospective analysis (with ethical approval)in Al-Dhaid teaching hospital-Sharjah/UAE from June 2015 till October 2015. Swab from vagina and another from rectum is taken from all pregnant ladies attending antenatal care (1000 pregnant lady), swab from both sit placed into a selective (enrichmentbroth) media which encourage the growth of GBS. If bacteria growth in the broth it will be considered as positive.Urine test done to those in doubt of vagina GBS but negative test.So we divided the patient admitted through antenatal care to labour room to screened and unscreened patient and we compare the neonatal outcome of both. The pregnant women attend at 34-35 week and the swab is taken for culture and sensitivity those women are compared with other women attending to labour room or antenatal care without screening for a reason or another. Those with positive screening test covered with penicillin doses in the first stage of labour (8-10 hours)before labour. Results:- In this study: 1. Incidences of screened patient are much higher than unscreened patient throughout the months 2. Incidence of hospital stay and receiving unnecessary antibiotics are much less in screened than unscreened 3. Financial burden in screened patient are much less than unscreened because of less hospital stay, less antibiotics use, less neonatal stay in SCABU 4. Our incidence of infected women among screened group is 25.7% 5. 1-2% of neonatal got infected in unscreened compared to 0.02% in screened with 3% got complicated in unscreened ladies compared to 0.01% in screened. Conclusion: Screening of GBS in pregnant ladies with antibiotics cover prior to labour by normal vaginal delivery reduce perinatal mortality and morbidity.