The hydroalcoholic extract of leaves of Ajuga bracteosa (Ratpatiya) at the rate of 10 mg/100 gm body weight was tested as antidiabetic for hypoglycemic activity in alloxan induced diabetic chicks. Alloxan was used to induce diabetes mellitus in chicks and the blood glucose level were estimated by using commercial kit (Span Biotech, Surat, India). After the confirmation of diabetes in chicks, hydroalcoholic extract of leaves of Ajuga bracteosa was administered orally to the diabetic chicks for 30 days daily. The present investigation revealed that a significant reduction of blood glucose levels was observed in diabetic chicks treated with leaves of Ajuga bracteosa as an antidiabetic therapy, although the blood glucose level was observed higher in diabetic control chicks (p<0.05). However, a high significant value of blood glucose level was found in diabetic control chicks as compared to normal control chicks. Hence, our study shows that the hydroalcoholic extract of leaves of Ajuga bracteosa has the antidiabetic potency which reduces the blood glucose level in diabetic chicks.