Malondialdehyde (MDA) as a biomarker is widely used for examining oxidative stress in biomedical field where Lipid peroxidation is a chain phenomenon resulting in the formation of various active compounds that result in cellular damage. Bio monitoring of MDA is used as a biomarker in different diseases like in-vivo and in vitro. MDA is also used in various types of diseases like hypertension, diabetes, heart failure and cancer etc. Higher level of MDA was found in patients that suffer from various types of diseases including lung cancer, complex original pain syndrome. In finding the oxidative stress MDA is used as a reliable biomarker. MDA is found in high levels in patients that suffer with goiter. MDA determination can take place by different methods. The determination of MDA was studied in goiter patients through a simple, and rapid but sensitive scientific method. This review is aimed to study the biochemical mechanism of formation of maliondialdehyde and the role of Maliondialdehyde as a biomarker for the diagnosis of several biochemicalcompetition.