AbstractIntroduction:- Infections caused by MRSA are worldwide, detecting the mecA gene or its product by PCR isrecognized as a gold standard for detection of MRSA. In resource limited clinical settings phenotypic methodwhich is simple, rapid, accurate and cost effective is required. Cefoxitin disc diffusion is considered as surrogatemarker for mecA gene, and could be considered as gold standard for MR isolates. MRSA infection is of concernbecause it is resistant to a number of widely used antibiotics. Treatment options for MRSA are limited and lesseffective, than options available for susceptible S. aureus infections leading to increased morbidity and mortalityin hospitalized patients. To control MRSA in hospitals, correct antibiotic treatment in infected patients is requiredand prevent their spread.Object: This study is conducted to know the resistance pattern of various antibiotics in Methicillin-resistantStaphylococcus aureus(MRSA)Material and Methods:Type of study: Cross-sectional prospective analytical studyStudy time: November 2012 to April 2014Sample size: Total of 174 S. aureus isolated from non-repetitive clinical samples from IPD and OPD of tertiarycare hospital in Bhopal.Result: 174 Staphylococcus aureus strains isolated from the non-repetitive clinical samples were processed forMRSA identification. Out of 174 S. aureus isolates 69(39.65%) were found to be MRSA by cefoxitin discdiffusion test and rest 105 strains were MSSA. Among MRSA more than 70% resistance is for Ampicillin andErythromycin and low resistance was for Netilmycin 27.53%, Doxycycline 24.63% and 5.79% for Linezolid andno resistance for Teicoplanin and Vancomycin.Discussion: Multidrug resistance among S. aureus is a potential threat for the health care settings. Prolongedhospitalization and antibiotic therapy especially with β-lactam antibiotics predispose patients to the acquisition ofMDR. To control and prevent the spread of MRSA in hospitals, correct antibiotic treatment in infected patientsis required and ad mistered.

AbstractIntroduction:- Infections caused by MRSA are worldwide, detecting the mecA gene or its product by PCR isrecognized as a gold standard for detection of MRSA. In resource limited clinical settings phenotypic methodwhich is simple, rapid, accurate and cost effective is required. Cefoxitin disc diffusion is considered as surrogatemarker for mecA gene, and could be considered as gold standard for MR isolates. MRSA infection is of concernbecause it is resistant to a number of widely used antibiotics. Treatment options for MRSA are limited and lesseffective, than options available for susceptible S. aureus infections leading to increased morbidity and mortalityin hospitalized patients. To control MRSA in hospitals, correct antibiotic treatment in infected patients is requiredand prevent their spread.Object: This study is conducted to know the resistance pattern of various antibiotics in Methicillin-resistantStaphylococcus aureus(MRSA)Material and Methods:Type of study: Cross-sectional prospective analytical studyStudy time: November 2012 to April 2014Sample size: Total of 174 S. aureus isolated from non-repetitive clinical samples from IPD and OPD of tertiarycare hospital in Bhopal.Result: 174 Staphylococcus aureus strains isolated from the non-repetitive clinical samples were processed forMRSA identification. Out of 174 S. aureus isolates 69(39.65%) were found to be MRSA by cefoxitin discdiffusion test and rest 105 strains were MSSA. Among MRSA more than 70% resistance is for Ampicillin andErythromycin and low resistance was for Netilmycin 27.53%, Doxycycline 24.63% and 5.79% for Linezolid andno resistance for Teicoplanin and Vancomycin.Discussion: Multidrug resistance among S. aureus is a potential threat for the health care settings. Prolongedhospitalization and antibiotic therapy especially with β-lactam antibiotics predispose patients to the acquisition ofMDR. To control and prevent the spread of MRSA in hospitals, correct antibiotic treatment in infected patientsis required and ad mistered.