The present study explores the dynamics of borders, focusing on the complex cultural and historical interaction between Jaguarão, Brazil, and Rio Branco, Uruguay. The main objective is to discuss the role of borders as multidimensional spaces that influence social, cultural, and economic interactions, highlighting their duality as barriers (real/imaginary) and transition zones that shape territorial organization and local identities. It examines how borders address the challenges posed by globalization and how city twinning can facilitate international cooperation and cultural exchanges. Using a qualitative methodology, this analysis is based on a literature review of recent articles, theses, and dissertations, complemented by classic and contemporary historical sources on the topic of borders and twin cities. The results show that despite the challenges posed by globalization and border strengthening policies, cities like Jaguarão and Rio Branco exemplify how borders can be permeable and dynamic, facilitating cooperation and cultural exchange. It concludes that borders are transition zones that offer opportunities for the development of more integrated urban policies and economic strategies, promoting a more inclusive and supportive globalization. This study highlights the importance of rethinking borders not as barriers, but as bridges for cooperation and cultural coexistence.