Background: There are several modalities for the treatment of vitiligo but generally they do not result in significant cure of the disease. Autologous non-cultured non trypsinised epidermal cell suspension is simple, less time consuming and does not required special setup. Aim: To evaluate the Efficacy of Autologous Non-cultured non-trypsinised melanocyte-keratinocyte cell transplantation in treatment of stable vitiligo patches. Materials and Methods: Patient of age 18 years and above with stable vitiligo, showing no expansion of pre-existing lesion since last 6 months and who are resistant to medical therapy treated with Autologous Non-cultured non-trypsinised melanocyte-keratinocyte cell transplantation technique. Results: Excellent repigmentation (extent of repigmentation of >75%) response showed by 30% cases, Very good response (extent of repigmentation of 50%-75%) in 46%, Good response (extent of repigmentation of 25-49%) in 16%, Poor response (repigmentation <25%) in 6% at the end 20 weeks post-surgery and good colour match with surrounding pigmented area. Conclusion: Noncultured nontrypsinized melanocyte keratinocyte transplantation technique is easier, cheaper, less time consuming, less expertise needed and can cover larger body surface area with smaller grafts. This technique produces uniform and cosmetically acceptable pigmentation.

Background: There are several modalities for the treatment of vitiligo but generally they do not result in significant cure of the disease. Autologous non-cultured non trypsinised epidermal cell suspension is simple, less time consuming and does not required special setup. Aim: To evaluate the Efficacy of Autologous Non-cultured non-trypsinised melanocyte-keratinocyte cell transplantation in treatment of stable vitiligo patches. Materials and Methods: Patient of age 18 years and above with stable vitiligo, showing no expansion of pre-existing lesion since last 6 months and who are resistant to medical therapy treated with Autologous Non-cultured non-trypsinised melanocyte-keratinocyte cell transplantation technique. Results: Excellent repigmentation (extent of repigmentation of >75%) response showed by 30% cases, Very good response (extent of repigmentation of 50%-75%) in 46%, Good response (extent of repigmentation of 25-49%) in 16%, Poor response (repigmentation <25%) in 6% at the end 20 weeks post-surgery and good colour match with surrounding pigmented area. Conclusion: Noncultured nontrypsinized melanocyte keratinocyte transplantation technique is easier, cheaper, less time consuming, less expertise needed and can cover larger body surface area with smaller grafts. This technique produces uniform and cosmetically acceptable pigmentation.