This repository contains two large annotated flow cytometry datasets (BLAST110, LAIP29) for AML and healthy control patients used in Mocking et al. The BLAST110 cohort was gated to identify immature myeloid blasts. The LAIP29 cohort comprises different patients and was gated to also identify leukemic (LAIP+) populations. Scripts for reproducing our analyses are available at: https://github.com/AUMC-HEMA/cMRD-manuscript Datasets Each dataset is structured contains the following two folders /FCS/ contains raw .fcs files /labels/ contains .csv files containing cell-specific annotations The .csv files and .fcs files share a common parameter ("event_ID") which can be used to link the cell labels to each FCS file. The LAIP29 contains an additional file folders /annotations/. This shows the characterization and abundance of each leukemic populations. BLAST110 /BLAST110 contains FCS files from 110 different AML and healthy control samples: 20 normal bone marrow (NBM) samples 30 AML diagnosis ("Dx") samples 90 AML follow up ("FU") samples Collected at different timepoints after induction chemotherapy Different levels of measurable residual disease (MRD) The breakdown of this available in sample_info.csv LAIP29 /LAIP29 contains FCS files from 29 different AML samples measured at diagnosis and follow up. Each sample can have matching diagnosis and follow up timepoints. This information is available in sample_info.csv Manual gating BLAST110 In the BLAST110 cohort, was manually gated according to the following gating hierarchy: WBC based on FSC-A, SSC-A WBC/Singlets based on FSC-A, FSC-H WBC/Singlets/CD45pos based on SSC-A, CD45 WBC/Singlets/CD45pos/Lymphocytes based on SSC-A, CD45 WBC/Singlets/CD45pos/CD45dim based on SSC-A, CD45 WBC/Singlets/CD45pos/CD45dim/CD34pos based on SSC-A, CD34 WBC/Singlets/CD45pos/CD45dim/CD117pos based on SSC-A, CD117 The "Blast" label combines the ouptut of CD34pos and CD117pos LAIP29 The LAIP29 cohort was gated according conventional MRD analysis by experts and was annotated similarly to the BLAST110 cohort, with added detail for leukemic cells. The LAIP column indicates whether a cell was gated as LAIP+. Each unique leukemic population was given a unique identifier LAIP_ID. Because overlap between LAIPs is not taken into account during gating, each leukemic cell can belong to multiple LAIPs, resulting in a string (e.g., "1" for only LAIP 1, "1_2_3" for a cell positive for 3 LAIPs).