Fig. 1. Eschaneustyla terricola (A, B) and E. lugeri (C–E) during interphase, and E. lugeri during morphogenesis (F–L), after protargol impregnation. (A, B) Ventral and dorsal view of same specimen, showing ciliature. Cirri formed from same anlage were connected by solid line. Asterisk denotes the distal end of AZM. (C) Schematic representations of midventral-complex of four E. lugeri specimens with two long midventral rows. Arrows and arrowheads denote midventral rows 1 and 2, respectively. (D, E) Ventral and dorsal view of same specimen, showing typical infraciliature. Cirri formed from same anlage were connected by solid line. Two leftmost cirri develop from anterior part of undulating membranes anlage (dotted line). (F) Ventral view of an early divider that twisted a little at the anterior end, to show the oral primordia of proter (arrow) and opisthe (arrowhead). (G) Enlarged image of proter's oral primordium (arrow) and undulating membranes anlage (arrowhead) of the individual shown F. Note that the former develops in deep of the cortex while the latter develops in the surface. (H) Ventral view of an early divider to show the frontoventral cirral anlagen (arrows). Arrowhead denotes the penultimate frontoventral cirral anlage which forms midventral row 1. Double-arrowhead denotes the frontoventral cirral anlage n that develops into frontoterminal row. Dashed circle represents the flurry area due to the hyperchromatism after protargol impregnation. (I, J) Ventral and dorsal view of an early divider to show the frontoventral cirral anlagen. Arrows and arrowheads in I mark the penultimate and the last frontoventral cirral anlagen, respectively. Note that marginal anlagen and dorsal kineties anlagen (arrows in J) develop intrakinetally. Macronuclear nodules are fusing (arrowheads in J). (K) Ventral view of a middle divider. Arrow marks the partly renewed adoral zone of membranelles of proter. Asterisk denotes two leftmost frontal cirri developed from the undulating membranes anlage. L. Nuclear apparatus of the same specimen as shown in K. Note that macronuclear nodules are fusing into a single mass. AZM, adoral zone of membranelles; CC, caudal cirri; E, endoral; FT, frontoterminal row; LMA, left marginal anlage; LMR, left marginal row; Ma, macronuclear nodules; Mi, micronuclei; MV, midventral row; MV 1, midventral row 1; 1–4, dorsal kineties 1–4; P, paroral; RMA, right marginal anlage; RMR, right marginal row. Scale bars: 100 μm (A, B, D, E); 70 μm (F–L).

Published as part of Deng, Yufan, Lu, Xiaoteng, Li, Jingbao, Ba, Sang & Da Silva Paiva, Thiago, 2018, Morphology of Two Eschaneustyla Species (Ciliophora, Urostylida), with Notes on Morphogenesis of Eschaneustyla lugeri, pp. 69-78 in Acta Protozoologica 57 (2) on page 71, DOI: 10.4467/16890027AP.18.007.8981, http://zenodo.org/record/10994408