Earth Movers Distance (aka Wasserstein distance) is a way of describing the difference between two distributions. It is a more accurate way of demonstrating differences in cytometric datasets than metrics such as delta-MFI, which neglect the single-cell nature of these datasets. This version of the code is aimed at mass cytometric datasets, so includes an arcsinh transformation. EMD is a non-directional measurement of difference, so the directionality is added by comparing the means of the datasets. This can be changed to median or another metric if needed.