3DED data of commerically obtained L-histidine monohydrochloride monohydrate were collected using continuous rotation in selected area mode. The table below summarizes generic data collection parameters for the data collections. Project Project label Sample label NEDFSO_0023 Collection site University of Southampton, NCS Instrumental Instrument Rigaku XtaLAB Synergy-ED, electron diffractometer Radiation source LaB6 Accelerating voltage [kV] 200 Wavelength [Å] 0.02510 Probe type Parallel beam Beam diameter Beam convergence Parallel beam Detector Rigaku HyPix-ED, hybrid pixel array detector Number of pixels in the image 775 x 385 Pixel size [µm] 100 Hardware binning 1 Sample description Name L-histidine monohydrochloride monohydrate Chemical composition C6H9N3O2 · HCl · H2O Sample source commercial Grid Agar Scientific holey carbon coated copper grid 200 mesh Sample preparation solid sample ground with mortar and pestle, dispersed between microscopy glass slides, grid tapped on solid, cryo transfer @ 175 K Sample holder GATAN Elsa cryo-transfer holder 698 Experimental Data type Electron diffraction data - 3DED Data collection method continuous rotation - cRED Collection temperature [K] 175 Software Software used for the data collection CrysAlisPro 1.171.43.112a Software used for processing CrysAlisPro 1.171.43.119a Files and data formats Image folders ./frames/xxx.rodhypix./frames/PETS_exp_705/frames/xxx.tiff Image format .rodhypix (Rigaku proprietary image format).tiff (16bit, converted with CrysAlisPro 1.171.43.119a) Additional files CrysAlisPro input files:./expinfo/./CrysalisExpSettings.ini./exp_705.par./exp_705.run./new.ccd PETS2 (http://pets.fzu.cz/) input file:./frames/PETS_exp_705/exp_705.pts2 Crystal Image:./exp_705_microed_grain_snapshot.jpg