This is a subset of the Supporting data for Guy M Hagen, Justin Bendesky, Rosa Machado, Tram-Anh Nguyen, Tanmay Kumar, Jonathan Ventura, Fluorescence microscopy datasets for training deep neural networks, GigaScience, Volume 10, Issue 5, May 2021, giab032, https://doi.org/10.1093/gigascience/giab032The selected subset contains 79 images from Data Set 4 in the form of a single tiff file. The paper describing the original dataset is available here: https://academic.oup.com/gigascience/article/10/5/giab032/6269106The original dataset is available here: http://gigadb.org/dataset/100888 AI4Life has received funding from the European Union’s Horizon Europe research and innovation programme under grant agreement number 101057970. Views and opinions expressed are however those of the author(s) only and do not necessarily reflect those of the European Union or the European Research Council Executive Agency. Neither the European Union nor the granting authority can be held responsible for them.