
This report presents a guide to selecting high-quality commercial antibodies against Long-chain-fatty-acid--CoA ligase 5 (ACSL5) by western blot, immunoprecipitation and immunofluorescence, using a standardized experimental protocol comparing read-outs in knockout cell lines and isogenic parental controls. This study was funded in part by the ALS-Reproducible Antibody Platform (ALS-RAP), established as a public-private partnership by three prominent ALS charities - the ALS Association (USA), the Motor Neurone Disease Association (UK), and the ALS Society of Canada.
ACSL5, Q9ULC5, western blot, antibody characterization, Amyotrophic Lateral Sclerosis, Long-chain-fatty-acid--CoA ligase 5, immunofluorescence, antibody validation, immunoprecipitation
ACSL5, Q9ULC5, western blot, antibody characterization, Amyotrophic Lateral Sclerosis, Long-chain-fatty-acid--CoA ligase 5, immunofluorescence, antibody validation, immunoprecipitation
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