Background: The most prevalent hospital-acquired illness among patients in a critical care unit using mechanical ventilation is ventilator-associated pneumonia. The nurses in Intensive Care Units have been shown to be in the best position to apply their knowledge into practice since they provide nursing care to patients at their bedsides around-the-clock, which makes them crucial in preventing ventilator-associated pneumonia (VAP).This study aimed to assess the adherence of health care workers to ventilator associated pneumonia bundle intervention. Methods: A quantitative cross-sectional study was conducted using structured questionnaire among 50 health care workers working in the Intensive Care Units (ICU) of P.T. Birta City Hospital and Research Center Pvt. Ltd. Data was analyzed in terms of frequencies and percentage using statistical package for social sciences (SPSS version 23). Results: Eighty percent of health care workers in the study demonstrated acceptable understanding and perception of ventilator associated pneumonia and VAP bundle interventions in accordance with hospital policy/guideline, and the total mean compliance rate for VAP bundle implementation was 91%. Conclusion: There was acceptable level of adherence to the guidelines for preventing ventilator-associated pneumonia (VAP) in the intensive care units, however further observational studies are still required to assess the health care workers' adherence.