Physalis angulata AH-ZE1 form the family Solanaceae. Physalis angulata is considered a plant medically effective for the treatment of many diseases. The ability of Physalis to remove the reactive oxygen species ROS, strengthen the body's antioxidant system constitutes its defense mechanism. The results of the Gas Chromatography–Mass Spectrometry ( GC-MS) analysis showed that the sample Physalis angulata isolate AH-ZE1 contained twenty-four effective compounds that have a lot of medicinal efficacy. Antioxidant content of 94% at the concentration of 140 µg / ml of the extract, and the lowest percentage of antioxidant 42% at the concentration of 20 µg / ml is as a result of the high percentage of phenolic compounds in the plant extract, which is directly proportional to the percentage of antioxidants.