This study aims to determine the role of Work Life Balance in mediating the effect of Workplace Flexibility on Job Satisfaction. The research was conducted on Education Personnel both civil servant status and contracts at the Head Office of Udayana University with a sample of 181 Education Personnel. Sample determination using proportionate random sampling. The data was analyzed using Structural Equation Modeling (SEM) with a Partial Least Square (PLS) approach. The results showed that workplace flexibility has a positive and significant effect on job satisfaction, work life balance has a positive and significant effect on job satisfaction, workplace flexibility Positively and significantly affect work-life balance, and work-life balance is able to mediate the effect of workplace flexibility on job satisfaction. These results illustrate that to increase employee job satisfaction at work, work unit leaders must provide good Workplace Flexibility that can improve Work Life Balance.