This is a brochure showcasing recent use cases on EOSC and Open Science The brochure presents 31 use cases from different countries in Europe on policies and practices to implement EOSC and Open Science at national level with a short description, key facts, and links for further information for each use case The use cases have been collected from Member States and Associated Countries in the EOSC Steering Board via the Survey on National Contributions to EOSC and follow-on interviews with designated representatives for each use case The annual results of the Survey on National Contributions to EOSC are publicly available in the online dashboard of the EOSC Observatory: [https://eoscobservatory.eosc-portal.eu] This brochure has been developed by the EOSC Future project