Abstract: This talk will cover how the predictions submitted by participants in CACHE Challenge #1, targeting LRRK2, were tested at the CACHE Experimental Hub. The Hub, housed in the SGC Toronto’s laboratories, has the expertise and capacity to conduct a broad spectrum of biophysics and biochemistry experiments amenable to several protein families. The main methods used in CACHE Challenge #1 were surface plasmon resonance, dynamic light scattering, 19F-nuclear magnetic resonance, and differential scanning fluorimetry, all of which will be discussed in the context of the released data. After training in analytical chemistry at McMaster University, Suzanne gained industry experience at Sanofi, Sciex and a proteomics start-up. In 2014, Suzanne joined the SGC in Toronto as a Project Manager to facilitate chemical probe development partnerships with big pharma. Suzanne was the Project Manager for CACHE Challenge #1.