This data set contains two, 40-minute videos of the mouse face while the LEDs of the imaging system are strobing every other minute. One video is taken with the aluminum hemisphere removed from the imaging system while the other video is taken with the aluminum hemisphere installed on the imaging system as usual. A trigger file recorded by the data acquisition system shows the timing of measurements. This data was used to produce figure 3 in the following article. Doran, P., Fomin-Thunemann, N., Tang, R., Zimmerman, B., Kilic, K., Kura, S., Fisher, H., Chabbot, G., Herbert, J., Jiang, J., Sakadzic, S., Boas, D., Devor, A., Chen, I., & Thunemann, M. (2023). Widefield in vivo imaging system with two fluorescence and two reflectance channels, a single sCMOS detector, and shielded illumination. bioRxiv. https://doi.org/https://doi.org/10.1101/2023.11.07.566086do