Extracellular vesicles (EVs) constitute a diverse array of membrane-bound nanoparticles emitted by different cell types. They possess the capacity to convey molecular signals and transport proteins, messenger RNAs (mRNAs), and non-coding RNAs like microRNAs (miRNAs) from donor to recipient cells, thereby impacting cellular responses and bioactivity. Transmission electron microscopy stands as the definitive method for imaging these nanoparticles due to its unparalleled resolution and capacity to discern minute structural details.