This GitHub release accompanies our paper, "A unified approach to evolutionary conservation and population constraint in protein domains highlights structural features and pathogenic sites," accepted in principle by Communications Biology. It provides the version of the code used for analyses and figure generation in the study. Includes: Core Dataset: Aggregated statistics from Pfam-A, gnomAD, ClinVar, and PDBe databases. Notebooks for Analysis: R notebooks for statistical computations and figures as detailed in the paper. Manuscript Figures Generation: Scripts to reproduce manuscript figures. Citation: Refer to our paper for methodology and findings: Stuart A. MacGowan, Fábio Madeira, Thiago Britto-Borges and Geoffrey J. Barton, "A unified analysis of evolutionary and population constraint in protein domains highlights structural features and pathogenic sites." Pre-print available at https://doi.org/10.21203/rs.3.rs-3160340/v1. License: Released under the MIT license by Stuart A. MacGowan and contributors at the University of Dundee. Feedback and contributions are welcomed to refine and enhance this framework.