Abstract The utilization of plant extracts, particularly Tulsi (Ocimum tenuiflorum), in the biological control of mosquitoes has garnered increasing attention due to its potential as a natural and sustainable approach to mosquito management. This review paper aims to critically examine the role of Tulsi extract in controlling mosquito populations and mitigating the transmission of mosquito-borne diseases. Through a comprehensive analysis of existing literature and research findings, the paper elucidates the biochemical properties of Tulsi extract relevant to mosquito control, including its chemical composition, mechanisms of action, and efficacy against different mosquito species. Furthermore, the paper explores the potential applications of Tulsi extract in integrated mosquito management strategies, considering factors such as larvicidal, adulticidal, and repellent activities, as well as its environmental impact and safety profile. By synthesizing current knowledge and research gaps, this review provides valuable insights into the utilization of Tulsi extract as a biocontrol agent for mosquitoes, highlighting its potential contributions to public health and sustainable vector control initiatives.