This paper presents the study of the effectiveness of horizontal transfer of local isolates of the pathogenic fungus Beauveria bassiana (Balsamo) on adults of olive fruit fly Bactrocera oleae (Rossi) at a concentration of 10^6 spores/ml in laboratory conditions (this work was carried out in specialized scientific laboratories). In addition, it is not possible to reach the desired results in such experiments effectively when the data and observations of the study are not clear and accurate. For this reason, in this paper, experimental data will be presented with inaccurate or uncertain observations using neutrosophic statistics. The purpose is to know the success of males contaminated with pathogenic isolates in the transmission of infection to females. In laboratory conditions through a neutrosophic reading of the study data. This proposed presentation provides greater accuracy, flexibility, and applicability than the classic experimental design in the case of uncertainty.