AbstractWound healing is a process of replacing the damage tissue caused by various factors, which involves a complex series of event. A fibrous protein called keratin is found in the top layer of skin, hair, and nails. It has an impact on wound healing process. The ability of keratin to aid in cell adhesion, proliferation, and tissue regeneration results in the provision of a biocompatible matrix for the regrowth and regeneration of damaged tissue. In this project work the main aim is to isolate the keratin from human hair and incorporate in a chitosan-based biofilm which is loaded with an antibacterial agent called clindamycin. Keratin provides a good stability against enzymatic degradation of body cell and clindamycin has an anti-microbial effect that together make the research work a best way to deliver this formulation for biomedical applications. This experimental study manages the isolated amount of by successfully incorporate it into the chitosan-based biofilm. This article manages about the properties, categorization, and structure of keratin by FTIR analysis and SEM. The extraction of keratin is carried out by using chemical precipitation method and on the other hand, the chitosan-based biofilm is made by solvent evaporation method.KeywordsBiofilm, Chitosan, Clindamycin, FTIR analysis, Keratin Nano particle, NDDS.