Background: Electronic commerce have been instrumental to the growth of businesses globally even though it has its own challenges and setbacks Aims: This study assessed the impact of e-commerce on investments in Micro and Small Enterprises Calabar Metropolis. Specifically, it examined the impact of business to business commerce on investments in micro and small enterprises in Calabar Metropolis. Methodology: The study adopted the survey design. The population of the study was 320 micro and small enterprises in Calabar Metropolis, the instrument of data collection was the questionnaire and the data collected was analyzed using the tobit regression technique. Results/Findings: Findings from the Tobit regression showed that business to business commerce significantly impact on investments in micro and small enterprises in Calabar Metropolis. Thus, e-commerce influences investments in micro and small enterprises in Calabar Metropolis. Conclusions: From the findings of the study, it is concluded that in order for the micro and small enterprises in Calabar Metropolis to continue to experience improve investments, there should be improvements in internet services by the service providers so that network issues can be reduced to the barest minimum. This will go a long in speeding up business transactions between firms thus helping to improve sales, profits, and investments in the micro and small enterprises in Calabar Metropolic, Cross River State and Nigeria in general.